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Carl Zeiss
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Image Search Results
Journal: Scientific Reports
Article Title: Role of areca nut induced JNK/ATF2/Jun axis in the activation of TGF-β pathway in precancerous Oral Submucous Fibrosis
doi: 10.1038/srep34314
Figure Lengend Snippet: ( a ) Immunoblot representing compromise in areca nut induced pJNK levels in the presence of muscarinic acid receptor inhibitor (atropine) at 0.5 hours. ( b ) Representative images from depicting areca nut induced calcium release. White arrows depict the cells with higher calcium levels post areca nut treatment. Magnification factor; 10X, Scale bar = 100 μm. ( c ) Immunoblot representing compromise in areca nut induced pCAMKII levels in the presence of muscarinic acid receptor inhibitor (atropine) at 0.5 hours. ( d ) Immunoblot representing compromise in areca nut induced pJNK levels in the presence of CAMKII inhibitor (KN93) at 0.5 hours. ( e ) Representative images for live confocal imaging of areca nut induced ROS at 0 and 30 minutes. Magnification factor; 63X. ( f ) Immunoblot representing compromise in areca nut induced pJNK levels in the presence of ROS inhibitor (DPI) at 0.5 hours. β-actin is used as loading control in all the immunoblots.
Article Snippet: Dual staining for pATF2 and p-c-Jun was detected by
Techniques: Western Blot, Imaging
Journal: Scientific Reports
Article Title: Role of areca nut induced JNK/ATF2/Jun axis in the activation of TGF-β pathway in precancerous Oral Submucous Fibrosis
doi: 10.1038/srep34314
Figure Lengend Snippet: ( a ) Representative immunofluorescence images of areca nut induced nuclear localized pATF2 and p-c-Jun at 2 hours. Magnification: 63X; Zoom: 1.8; Scale bar = 10 μm. ( b ) Bar graph of qPCR results of chIP assay showing fold enrichment of pATF2 and p-c-Jun on TGF-β2 promoter at 2 hours upon areca nut treatment compared to untreated cells. ( c ) Bar graph showing fold change at 2 hour of areca nut induced TGF-β2 and its compromise upon transient knock down of ATF2 or c-Jun. ( d,e ) Immunoblots representing pSMAD2 levels at 2 hour areca nut treatment and its compromise upon transient knock down of ATF2 or c-Jun. β-actin is used as loading control in all the immunoblots. *** , ** represent p values ≤ 0.0001; 0.001 respectively. (5H; 5 μg/ml areca nut extract).
Article Snippet: Dual staining for pATF2 and p-c-Jun was detected by
Techniques: Immunofluorescence, Western Blot